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【J Vasc Surg】IL-1受体拮抗剂降低了兔脊髓缺血性
Interleukin-1 receptor antagonist attenuates the severity of spinal cord ischemic injury in rabbits
Satoshi Akuzawa, MD, Teruhisa Kazui, MD, PhD, Enyi Shi, MD, PhD, Katsushi Yama***a, MD, PhD, Abul Hasan Muhammad Bashar, MBBS, PhD, Hitoshi Terada, MD, PhD
Received 10 January 2008; accepted 6 April 2008. published online 24 June 2008.
Objective
Thoracic and thoracoabdominal aortic surgery is sometimes complicated by subacute or delayed paraplegia. Pro-inflammatory cytokine interleukin-1 (IL-1) β has been implicated in extensive inflammation and progressive neurodegeneration after ischemia. Using a rabbit model, we investigated the neuroprotective effects of IL-1 receptor antagonist (IL-1ra) in a temporal fashion.
Methods
Spinal cord ischemia was induced by aortic cross-clamping in New Zealand White rabbits. The animals were assigned to three groups. Group C (n = 20) received saline (0.2-mL) and Group I (n = 20) received IL-1ra (200-μg/0.2-mL) intrathecally just after reperfusion. Group S (n = 3) underwent sham operation without aortic occlusion. We assessed the neuroprotective effects of IL-1ra by evaluating neurological function, histopathological changes, and in-situ terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL staining). We also measured the levels of Nitric Oxide (NO) and S100β in cerebrospinal fluid (CSF). Each evaluation was performed sequentially within 120 hours after reperfusion.
Results
Group C showed progressive deterioration of motor function which became statistically significant from 48 hours after the onset of reperfusion (P < .05, P < .01, P < .001, P < .001 at 48, 72, 96, and 120 hours, respectively). Compared to Group C, a higher number of viable neurons was observed with less severe spinal cord injury in Group I (P < .01, .05 and .05 at 24, 72, and 120 hours, respectively). TUNEL-positive neurons were also significantly reduced by the administration of IL-1ra (P <.01 and .05 at 24, and 120 hours, respectively). The difference between Group C and Group I with regard to NO was significant at 72 and 120 hours (P < .05), while that in terms of S100β was significant only at 24 hours (P < .05).
Conclusions
Administration of IL-1ra attenuates spinal cord ischemic-reperfusion injury as evidenced by reducing both neuronal necrosis and apoptosis 本人认领，若48小时未上交译文则视为放弃，请其他战友继续。 Interleukin-1 receptor antagonist attenuates the severity of spinal cord ischemic injury in rabbits
IL-1受体拮抗剂可降低兔脊髓缺血性损伤的严重 IL-1受体拮抗剂可降低兔脊髓缺血性损伤的严重
目的
胸部外科和胸腹主动脉外科手术有时会伴发亚急性或延迟性截瘫。促炎症反应细胞因子白介素（IL-1)β涉及缺血后广泛的炎症反应和进行性神经退行性变。使用兔模型，我们研究了IL-1受体拮抗剂(IL-1ra)颞叶部位的神经保护作用。
方法
通过钳夹新西兰白色大鼠主动脉建脊髓缺血模型。动物分三组。再灌注后，组C (n = 20)鞘内注射盐水 (0.2-mL) ，组I (n = 20) 鞘内注射IL-1受体拮抗剂 (200-μg/0.2-mL) .组S
（n=3）主动脉为阻断而行假手术。我们通过测量神经系统功能，组织病理学变化，原位脱氧核酸转移酶介导的dUTP生物素切口末端标记(TUNEL染色)来评价IL-1受体拮抗物的神经保护作用。我们也测量了脑脊液中（CSF）的一氧化氮浓度（NO）和S100β。每次评估都在再灌注后连续120小时内进行。
结果
再灌注48小时后组C显示运动功能进行性衰退非常明显（48，72，96和120小时后分别P < 0.05, P < .01, P < .001, P < .001 ）。与组C相比较，在脊髓损伤较轻的组I可以观察到
更多的有活力的神经元（24，72和120小时分别为P < 0.01, p<0.05 和p<0 .05）。 注射IL-1受体拮抗剂后,TUNEL阳性神经元明显减少（24和120小时后p值分别为P <0.01 和p<0.05 ）。在72小时和120小时，组C和组I中NO明显不同(P<0.05), 而S100β只有在24小时时才具有统计学意义(P < 0.05).
结论
IL-1受体拮抗剂通过减少神经坏死和凋亡降低了兔脊髓缺血再灌注损伤。 哪位战友提供下全文，谢谢！
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作者:admin@医学,生命科学 2010-10-21 05:11
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