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【编译】《Oncogene》基于RNA干扰的哺乳动物细胞功
RNA-interference-based functional genomics in mammalian cells:
reverse genetics coming of age
Jose Silva1, Kenneth Chang1, Gregory J Hannon*1 and Fabiola V Rivas1
1Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
Sequencing of complete genomes has provided researchers
with a wealth of information to study genome organization,
genetic instability, and polymorphisms, as well as a
knowledge of all potentially expressed genes. The
identification of all genes encoded in the human genome
opens the door for large-scale systematic gene silencing
using small interfering RNAs (siRNAs) and short hairpin
RNAs (shRNAs). With the recent development of siRNA
and shRNA expression libraries, the application of RNAi
technology to assign function to cancer genes and to
delineate molecular pathways in which these genes affect
in normal and transformed cells, will contribute signifi-
cantly to the knowledge necessary to develop new and also
improve existing cancer therapy.
Oncogene (2004) 23, 8401–8409. doi:10.1038/sj.onc.1208176
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作者:admin@医学,生命科学 2011-04-06 14:04
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