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【bio-news】RNA干扰新途径
454 Sequencing?reveals new pathway in RNA interference
Application by Cold Spring Harbor Laboratory yields new insights on DNA methylation
BRANFORD, Conn., October 25, 2006 -- CuraGen Corporation's (NASDAQ: CRGN) majority-owned subsidiary 454 Life Sciences today announced that research on small RNAs, conducted by Cold Spring Harbor Laboratory, has revealed the dual role of the ARGONAUTE 4 protein (AGO4) in RNA-directed DNA methylation. The research, which describes a method for obtaining a comprehensive view of the total small RNAs from a single sample, was published today in Nature. The study, entitled, "Distinct Catalytic and Non-Catalytic Roles of ARGONAUTE4 in RNA-directed DNA Methylation," describes how 454 SequencingTM was used to elucidate the small RNAs associated with AGO4 and the AGO1 protein.
"Small RNAs have emerged as a means of genetic control that were completely unsuspected only 10 years ago. Virtually every area of biology has been affected by the discovery of these pathways. What we are now finding is that the diversity of small RNAs in many systems is even greater than previously suspected. The new study shows this in Arabidopsis (a model plant), where small RNAs have a major role in controlling the epigenetic organization of the entire genome," said Gregory Hannon of Cold Spring Harbor Laboratory, who led the research. "Conventional DNA sequencing methods gave us glimpses of the various classes of small RNAs that exist in plants and animals. However, only with 454 Sequencing have we been able to fully appreciate the extent of small RNA populations and the breadth of biological processes they might affect," Dr. Hannon noted.
"The Genome Sequencer 20TM system allows researchers to rapidly characterize all of the small RNAs in their samples," said Michael Egholm, Ph.D., Vice President, Molecular Biology, 454 Life Sciences. "454 Sequencing offers high quality, fast and accurate sequencing of small RNAs which has revolutionized research in the mechanisms of RNA interference."
The role of small RNAs is an exciting and rapidly growing field of research that is benefiting from technical advances in comprehensive and rapid sequencing. Small RNAs have several biological functions essential for cell growth and development. They regulate mRNA stability and protein synthesis, cause post-transcriptional gene silencing of transposons, viruses and transgenes, and regulate chromatin organization and genome structure. This is the second paper by Dr. Hannon on the use of 454 Sequencing to study small RNA to be published by Nature. In recent months, several other research teams using 454 Sequencing to study small RNA in plants, mammals, and viruses have had their findings published in Science, Nature Genetics, Journal of Virology, and Genome Research. Dr. Hannon has generously made the protocol for his method utilizing 454 Sequencing available for download at www.454.com.
The Genome Sequencer 20TM system (GS 20) is exclusively distributed for 454 Life Sciences by Roche Applied Science, a division of Roche Diagnostics. Visit the Roche Diagnostics website for more information on purchasing 454 products at http://www.roche-applied-science.com. 我先认领,晚上交
多谢参与，还请使用本版规范的认领词 454测序仪?RNA干扰机制的新途径
(美国)冷泉港实验室提出关于DNA甲基化新的见解。2006年10月25日BRANFORD, Conn.CuraGen公司的控股公司454生命科学公司宣布由(美国)冷泉港实验室指导的小RNAs实验研究表明：AGO4蛋白在依赖RNA的DNA甲基化过程中起双重作用。这项研究描述从单样本获得整个小RNAs全景的方法，今天在《Nature》杂志上发表。这项题"ARGONAUTE4蛋白在依赖RNA的DNA甲基化过程中的特殊催化和非催化作用"的研究描述了454测序仪技术怎样被用于解释小RNAs与AGO4和AGO1蛋白的关系。
小RNAs被用于基因控制的方法在10年前还备受怀疑。事实上，这些途径的发现已经影响了生物学的所有领域。我们现在正发现的是：在许多生物系统中小RNAs的差异比以前所预想的更多。(美国)冷泉港实验室的带头人Gregory Hannon说:"这项新的研究表明:"在一种模型植物Arabidopsis中,小RNAs控制至少一代的完全基因组表达改变的主要作用"。传统的DNA顺序分析方法仅仅能让我们短暂的看见存在于动植物中的小RNAs的不同的分类。Hannon博士说:"然而，454测序公司是唯一已经能鉴别小RNA全部种类的和它影响的生物学进程的公司"。
454生命科学公司分子生物学的副总裁Michael Egholm博士说:"基因组测序仪20TM 系统能让研究者快速鉴定样本中的所有小RNAs"。454测序仪提供高质量、快速、准确的测定小RNAs序列的方法,这改革RNA干扰机制的研究。
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作者:admin@医学,生命科学 2011-06-29 05:45
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