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【Cancer research】老鼠肾癌细胞或可抑制人类肿瘤
“这种疗法使用常规生物学方法，避免了传统化疗的毒副作用，为癌症治疗提供了一种新选择。”罗戈辛研究所负责人巴里·史密斯在公告中表示。
研究证实，把老鼠癌细胞注入由藻类提取物制成的胶囊并移植到癌症患者的腹部，能够减缓或阻止肿瘤生长。
在研究第一阶段，研究人员在30多位癌症晚期患者身上进行的试验证实了这种疗法的可靠性。在第二阶段，试验扩展到了早期结肠癌、胰腺癌和前列腺癌患者。
此外，研究人员还在40只自发患上前列腺癌、肝癌和乳腺癌的猫和狗身上进行了试验。结果显示肿瘤发展受到了抑制，在某些情况下，肿瘤出现坏死或消失，这些动物最终恢复了正常生活。
史密斯说：“研究结果显示这种方法不只是针对某一类特定肿瘤，老鼠细胞可用于治疗多种人类肿瘤，人类细胞也可用于治疗多种动物肿瘤。”
罗戈辛研究所的研究从研制上述胶囊开始，随后进行了临床前研究，并发现移植到癌症患者体内的动物癌细胞与患者的免疫系统处于隔绝状态，但仍能发挥作用。
“现有许多治疗方法具有局限性，只是专注于某一方面，无法从整体上治疗疾病。”参与研究的斯图尔特·苏博特尼克指出，这种新技术改变了疾病的发展进程，在治疗中利用了人体的天然防御机制。
相关论文1月25日发表于在线版的《癌症研究》（Cancer Research）上。（来源：新华网） Expand+Hydrophilic Agarose Macrobead Cultures Select for Outgrowth of Carcinoma Cell Populations That Can Restrict Tumor Growth
Abstract
Cancer cells and their associated tumors have long been considered to exhibit unregulated proliferation or growth. However, a substantial body of evidence indicates that tumor growth is subject to both positive and negative regulatory controls. Here, we describe a novel property of tumor growth regulation that is neither species nor tumor-type specific. This property, functionally a type of feedback control, is triggered by the encapsulation of neoplastic cells in a growth-restricting hydrogel composed of an agarose matrix with a second coating of agarose to form 6- to 8-mm diameter macrobeads. In a mouse cell model of renal adenocarcinoma (RENCA cells), this process resulted in selection for a stem cell–like subpopulation which together with at least one other cell subpopulation drove colony formation in the macrobeads. Cells in these colonies produced diffusible substances that markedly inhibited in vitro and in vivo proliferation of epithelial-derived tumor cells outside the macrobeads. RENCA cells in monolayer culture that were exposed to RENCA macrobead-conditioned media exhibited cell-cycle accumulation in S phase due to activation of a G2/M checkpoint. At least 10 proteins with known tumor suppression functions were identified by analysis of RENCA macrobead-conditioned media, the properties of which offer opportunities to further dissect the molecular basis for tumor growth control. More generally, macrobead culture may permit the isolation of cancer stem cells and other cells of the stem cell niche, perhaps providing strategies to define more effective biologically based clinical approaches to treat neoplastic disease. Cancer Res; 71(3); 725–35. ?2011 AACR
http://cancerres.aacrjournals.org/content/early/2011/01/21/0008-5472.CAN-10-2258 http://cancerres.aacrjournals.org/content/early/2011/01/21/0008-5472.CAN-10-2254
标题：Three-Dimensional Culture of Mouse Renal Carcinoma Cells in Agarose Macrobeads Selects for a Subpopulation of Cells with Cancer Stem Cell or Cancer Progenitor Properties
Abstract
The culture of tumor cell lines in three-dimensional scaffolds is considered to more closely replicate the in vivo tumor microenvironment than the standard method of two-dimensional cell culture. We hypothesized that our method of encapsulating and maintaining viable and functional pancreatic islets in agarose–agarose macrobeads (diameter 6–8 mm) might provide a novel method for the culture of tumor cell lines. In this report we describe and characterize tumor colonies that form within macrobeads seeded with mouse renal adenocarcinoma cells. Approximately 1% of seeded tumor cells survive in the macrobead and over several months form discrete elliptical colonies appearing as tumor cell niches with increasing metabolic activity in parallel to colony size. The tumor colonies demonstrate ongoing cell turnover as shown by BrdU incorporation and activated caspase-3 and TUNEL staining. Genes upregulated in the tumor colonies of the macrobead are likely adaptations to this novel environment, as well as an amplification of G1/S cell-cycle checkpoints. The data presented, including SCA-1 and Oct4 positivity and the upregulation of stem cell–like genes such as those associated with the Wnt pathway, support the notion that the macrobead selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties. Cancer Res; 71(3); 716–24. ?2011 AACR. 二医大欢迎您!广州中医药大学神经药理学教授李锐采用离体培训神经细胞方法,从神经分子水平研究“脑... (MEDcyber.com编译国外最新医学新闻) 头颈部肿瘤放疗易致甲状腺功能低下 (Cancer ...lib.shsmu.edu.cn/...anuary.htm-2002-01-24-快照-仁济医院图书馆Cervical cancer vaccine OK d 指导抗高血压药治疗家庭和动态血压作用相当 英医生首... 俄脑脊膜炎疫苗试验初获成功 美科学家用人类干细胞修复老鼠脊髓损伤 Total Lymphoid...www.renji.com/lib/view.asp-2011-01-19-快照-药学快讯(版权页)文件类型:PDF/Adobe Acrobat- 文字版科学国家实验室项目负责人和生命科学学院教授.据温龙平教授介绍,该成果是在实验大鼠 ... Growth in Nude Mice”,于近日发表在国际癌症研究的著名学术杂志 Cancer Research ...www.lib.gdpu.edu.cn/...839.pdf-2011-01-19-药学快讯(版权页)"[标签:content2]
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作者:admin@医学,生命科学 2011-01-28 08:47
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