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进展编译——Cell：癌细胞蛋白磷酸网络的单细胞
摘要：在磷酸化蛋白网络中改变生长因子的反应对于癌细胞的存活和病理非常重要。癌细胞信号网络的轮廓可能会确定细胞对于持续生长的环境线索的机制。使用多参数的流式细胞仪，在急性骨髓白血病中我们在单细胞水平上监测环境线索对于磷酸化蛋白的反应。通过暴露癌细胞信号网络在potentiating inputs中，而不是仅仅依靠蛋白磷酸化的基本水平，我们能够确认和遗传学、疾病演变相关联的唯一的癌网络轮廓。引人嘱目的是，单一的癌症显示唯一网络轮廓的多细胞亚型，在信号反应水平反应出癌症的不同质。这些结果显示，在癌细胞中信号网络剧烈地重构。以此，磷酸化蛋白反应的单细胞检测表明在磷酸化蛋白网络的信号潜能的转变，通过***的分子信号轮廓是细胞网络表型加以分类。
Cell Volume 118, Issue 2 , 23 July 2004, Pages 217-228
Article
Single Cell Profiling of Potentiated Phospho-Protein Networks in Cancer Cells
Jonathan M. Irish1, Randi Hovland2, 3, Peter O. Krutzik1, Omar D. Perez1, Øystein Bruserud3, 4, Bjørn T. Gjertsen3, 4 and Garry P. Nolan, 1, ,
1 Department of Microbiology & Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
2 Center for Medical Genetics and Molecular Medicine, Haukeland University Hospital and Proteomic Unit (PROBE), University of Bergen, Bergen, Norway
3 Department of Internal Medicine, Haematology Section, Haukeland University Hospital, Bergen, Norway
4 Institute of Medicine, Haematology Section, University of Bergen, Bergen, Norway
Received 10 January 2004; Revised 28 May 2004; accepted 28 May 2004 Published: July 22, 2004 Available online 22 July 2004.
Abstract
Altered growth factor responses in phospho-protein-driven signaling networks are crucial to cancer cell survival and pathology. Profiles of cancer cell signaling networks might therefore identify mechanisms by which such cells interpret environmental cues for continued growth. Using multiparameter flow cytometry, we monitored phospho-protein responses to environmental cues in acute myeloid leukemia at the single cell level. By exposing cancer cell signaling networks to potentiating inputs, rather than relying upon the basal levels of protein phosphorylation alone, we could discern unique cancer network profiles that correlated with genetics and disease outcome. Strikingly, individual cancers manifested multiple cell subsets with unique network profiles, reflecting cancer heterogeneity at the level of signaling response. The results revealed a dramatic remodeling of signaling networks in cancer cells. Thus, single cell measurements of phospho-protein responses reveal shifts in signaling potential of a phospho-protein network, allowing for categorizing of cell network phenotypes by multidimensional molecular profiles of signaling. 不知道在讲些什么。 较为准确的表达的确很重要，建议参与进展编译的战友从自己熟悉的领域开始，并在编译与交流的过程中逐渐扩大眼界，同时鼓励对个别文章深入研读，顺便提提自己的看法，从而深化个人对正在进行科学研究的认识，也加大丁香园交流的深度。 [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-03-30 15:14
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