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【文摘发布】过敏性小鼠的气道高反应性-气道关
The Role of Airway Closure
Author:Lennart K. A. Lundblad
Abstract:
Rationale: Allergically inflamed mice exhibit airway hyperresponsiveness to inhaled methacholine, which computer simulations of lung impedance suggest is due to enhanced lung derecruitment and which we sought to verify in the present study.
Methods: BALB/c mice were sensitized and challenged with ovalbumin to induce allergic inflammation; the control mice were sensitized but received no challenge. The mice were then challenged with inhaled methacholine and respiratory system impedance tracked for the following 10 minutes. Respiratory elastance (H) was estimated from each impedance measurement. One group of mice was ventilated with 100% O2 during this procedure and another group was ventilated with air. After the procedure, the mice were killed and ventilated with pure N2, after which the trachea was tied off and the lungs were imaged with micro-computed tomography (micro-CT).
Results: H was significantly higher in allergic mice than in control animals after methacholine challenge. The ratio of H at the end of the measurement period between allergic and nonallergic mice ventilated with O2 was 1.36, indicating substantial derecruitment in the allergic animals. The ratio between lung volumes determined by micro-CT in the control and the allergic mice was also 1.36, indicative of a corresponding volume loss due to absorption atelectasis. Micro-CT images and histograms of Hounsfield units from the lungs also showed increased volume loss in the allergic mice compared with control animals after methacholine challenge.
Conclusions: These results support the conclusion that airway closure is a major component of hyperresponsiveness in allergically inflamed mice.
Key Words: asthma • micro-computed tomography • input impedance • lung derecruitment • lung volume
AT A GLANCE COMMENTARY
Scientific Knowledge on the Subject
Airway hyperresponsiveness in mouse models of asthma has traditionally been attributed to contraction of airway smooth muscle. Recent studies suggest, however, that airway closure may play a more significant role.
What This Study Adds to the Field
Using the forced oscillation technique and micro-computed tomography, we now show that airway hyperresponsiveness in allergic mice can be largely explained by airway closure.
本期AJRCCM封面文章
http://ajrccm.atsjournals.org/cgi/content/short/175/8/768
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screen.width-333)this.width=screen.width-333" width=330 height=440 title="Click to view full coverfig.gif (330 X 440)" border=0 align=absmiddle> 发布翻译如下：（2007-4-14 11:38AM) were sensitized and challenged with ovalbumin to induce allergic inflammation似乎译为用卵蛋白致敏并激发来诱导产生过敏性炎症。因为致敏和激发用的都是卵蛋白。 致敏和激发是不同的，致敏的造模液含卵蛋白、氢氧化铝而激发仅用卵蛋白生理盐水悬液雾化吸入，我翻译时分开写也是为了避免混乱。 激发过程中气道粘液的分泌对气道阻力也是一个不容忽略的影响因素.
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作者:admin@医学,生命科学 2010-11-20 05:11
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