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【文摘发布】吸入氟替卡松对哮喘患者血管发生
Author:B N Feltis
Abstract:
Background: Subepithelial hypervascularity and angiogenesis in the airways are part of structural remodelling of the airway wall in asthma, but the effects of inhaled corticosteroids (ICS) on these have not been explored. Increased vascularity in asthma may contribute to a number of functional abnormalities. A study was undertaken to explore angiogenic modulation by ICS and its likely regulation via vascular endothelial growth factor (VEGF), its receptors and the angiopoietins.
Methods: A placebo-controlled intervention study with ICS in asthma was performed, examining vascularity, VEGF, its receptors (VEGFR1 and VEGFR2), and angiopoietin-1 (Ang1) to assess which of these factors were changed in the asthmatic airways after ICS treatment. Airway wall biopsy specimens, lavage fluid and cells were obtained from 35 patients with mild asthma randomised to receive ICS or placebo for 3 months, after which bronchoscopic examination and sample collection were repeated. Immunohistochemistry and image analysis were used to obtain quantitative measures of vessels, angiogenic sprouts, VEGF, VEGFR1, VEGFR2 and Ang1 staining in airway biopsy specimens. ELISA was used to assess VEGF concentrations in the lavage fluid.
Results: Vessel, VEGF and sprout staining were decreased after 3 months of ICS treatment. VEGF levels remained unchanged. VEGF receptors and Ang1 staining were not reduced after treatment.
Conclusions: The findings of this study support an effect of ICS in downregulating angiogenic remodelling in the airways in asthma, associated with decreasing VEGF activity within the airway wall. The environment of the airways after treatment with ICS, with changes in the balance between VEGF, its receptors, Ang1 and sprouts, appears to be less angiogenic than in untreated asthma.
Abbreviations: Ang1, angiopoietin-1; BAL, bronchoalveolar lavage; BHR, bronchial hyper-responsiveness; FP, fluticasone propionate; GMA, glycol methacrylate; ICS, inhaled corticosteroids; VEGF, vascular endothelial growth factor; VEGFR1, VEGFR2, vascular endothelial growth factor receptors R1 and R2
Thorax 2007;62:314-319
认领及参与讨论者方可pm我要全文 Title:Effects of inhaled fluticasone on angiogenesis and vascular endothelial growth factor in asthma
题目：吸入氟替卡松对哮喘患者的血管发生及血管内皮生长因子的作用吸吸入氟替卡松对哮喘患者血管发生及血管内皮生长因子的作用入氟替卡松对哮喘患者血管发生及血管内皮生长因子
Abstract:
Background: Subepithelial hypervascularity and angiogenesis in the airways are part of structural remodelling of the airway wall in asthma, but the effects of inhaled corticosteroids (ICS) on these have not been explored. Increased vascularity in asthma may contribute to a number of functional abnormalities. A study was undertaken to explore angiogenic modulation by ICS and its likely regulation via vascular endothelial growth factor (VEGF), its receptors and the angiopoietins.
摘要：
背景：气道上皮下血管过度形成及血管发生是哮喘患者气管壁结构重建的一部分，但是吸入型糖皮质激素（ICS）在这方面的作用并没有被研究。哮喘者血管过度生成可能导致一系列功能异常。通过ICS改变血管形成的研究正在进行，这很可能是通过血管内皮生长因子（VEGF）及其受体和血管生成素进行调节的。
Methods: A placebo-controlled intervention study with ICS in asthma was performed, examining vascularity, VEGF, its receptors (VEGFR1 and VEGFR2), and angiopoietin-1 (Ang1) to assess which of these factors were changed in the asthmatic airways after ICS treatment. Airway wall biopsy specimens, lavage fluid and cells were obtained from 35 patients with mild asthma randomised to receive ICS or placebo for 3 months, after which bronchoscopic examination and sample collection were repeated. Immunohistochemistry and image analysis were used to obtain quantitative measures of vessels, angiogenic sprouts, VEGF, VEGFR1, VEGFR2 and Ang1 staining in airway biopsy specimens. ELISA was used to assess VEGF concentrations in the lavage fluid.
方法：分为应用ICS治疗哮喘的治疗组及安慰剂治疗的对照组，检测血管形成，VEGF及其受体（VEGFR1 、VEGFR2）以及血管生成素-1 (Ang1)来评估其中哪些因子在哮喘患者应用ICS后发生改变。35例轻度哮喘患者被随机分为接受ICS治疗及安慰剂治疗组共治疗三个月，治疗前后进行支气管镜检查收集到气管壁活检标本、灌洗液及细胞学检查。应用免疫组化及图像分析方法对活检标本染色检测血管形成、血管芽生、VEGF, VEGFR1, VEGFR2和 Ang1，灌洗液中VEGF浓度检测采用ELISA方法。
Results: Vessel, VEGF and sprout staining were decreased after 3 months of ICS treatment. VEGF levels remained unchanged. VEGF receptors and Ang1 staining were not reduced after treatment.
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作者:admin@医学,生命科学 2010-10-28 17:11
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