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【编译】由成年体细胞所克隆的狗
一些哺乳动物，包括绵羊、小鼠、奶牛、山羊、猪、家兔、猫、骡子、马和一窝3只大鼠，通过将体细胞的细胞核转移到已经去核的卵母细胞中而被克隆。这项技术目前在狗身上没有获得成功，这是由于在体外培养犬的成熟卵母细胞存在着一定的困难性。在此我们报道将成年动物体细胞核转移至在体内已经成熟的卵母细胞中，从而克隆两只阿富汗猎犬的过程。结合由犬的基因组项目所产生的详细的序列信息，通过体细胞核转移的方法克隆狗的技术能够有助于判定遗传和环境因素对众多不同的犬类家系的不同的生物学和习性所施加的影响。
Nature 436, 641 (4 August 2005)
Dogs cloned from adult somatic cells
Byeong Chun Lee, Min Kyu Kim, Goo Jang, Hyun Ju Oh, Fibrianto Yuda, Hye Jin Kim, M. Hossein Shamim, Jung Ju Kim, Sung Keun Kang, Gerald Schatten and Woo Suk Hwang
Several mammals — including sheep, mice, cows, goats, pigs, rabbits, cats1, a mule2, a horse3 and a litter of three rats4 — have been cloned by transfer of a nucleus from a somatic cell into an egg cell (oocyte) that has had its nucleus removed. This technology has not so far been successful in dogs because of the difficulty of maturing canine oocytes in vitro. Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo. Together with detailed sequence information generated by the canine-genome project5, 6, the ability to clone dogs by somatic-cell nuclear transfer should help to determine genetic and environmental contributions to the diverse biological and behavioural traits associated with the many different canine breeds7, 8. [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-03-26 05:14
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