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【bio-news】Membrane microdialysis: Evaluation of a new met
题目：Membrane microdialysis: Evaluation of a new method to assess splanchnic tissue metabolism*
膜微透析：评价内脏组织代谢的一种新方法
摘要：
OBJECTIVE:Measuring peritoneal lactate concentrations could be useful for detecting splanchnic hypoperfusion. The aims of this study were to evaluate the properties of a new membrane-based microdialyzer in vitro and to assess the ability of the dialyzer to detect a clinically relevant decrease in splanchnic blood flow in vivo.
目的：腹膜内乳酸浓度测定有助于检出内脏血流灌注不足。本研究的目的是评价膜微透析仪的体外性质，并估计该透析仪在检测临床有关内脏血流量减少方面的在体工作能力。
DESIGN:: A membrane-based microdialyzer was first validated in vitro. The same device was tested afterward in a randomized, controlled animal experiment.
设计：先对膜微透析仪进行体外验证，然后再进行随机、对照动物实验。
SETTING:: University experimental research laboratory.
场所：大学的研究实验室。
SUBJECTS:: Twenty-four Landrace pigs of both genders.
对象：24只长白猪，雌雄兼用。
INTERVENTIONS:: In vitro: Membrane microdialyzers were kept in warmed sodium lactate baths with lactate concentrations between 2 and 8 mmol/L for 10-120 mins, and microdialysis lactate concentrations were measured repeatedly (210 measurements).
操作：体外，膜微透析仪在浓度分别为2 mmol/L和8 mmol/L的热乳酸钠浴中保持10～120min，并重复测定微透析乳酸钠的浓度（210次）。
In vivo: An extracorporeal shunt with blood reservoir and roller pump was inserted between the proximal and distal abdominal aorta, and a microdialyzer was inserted intraperitoneally. In 12 animals, total splanchnic blood flow (measured by transit time ultrasound) was reduced by a median 43% (range, 13% to 72%) by activating the shunt; 12 animals served as controls.
在体，在腹主动脉近端和远端之间连接一带血液收集器和旋转挤压蠕动泵的体外通路，并向腹膜内插入微透析仪。12只对照组动物在接入体外通路后内脏总血流量（循环时间超声测定）平均减少了43％（13%~72%）, [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-01-26 17:14
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