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【bio-news】可控制基因开关的新技术
From The TimesMarch 18, 2008
A new way of turning genes on and off, pioneered by a Nobel prize-winning British scientist, is promising to transform treatment of conditions such as HIV/Aids, heart disease and diabetes.
The technique, devised by Sir Aaron Klug, of the Laboratory of Molecular Biology in Cambridge, allows scientists to act with unprecedented precision against genes that affect a wide range of diseases, switching them on or off permanently.
The first drugs designed to target the genes have begun clinical trials in the United States on patients with arterial disease and diabetes-induced nerve damage. A third trial, for HIV, is due to begin within months. If they are successful, scientists predict that the technique could change the way many diseases are treated, making genetic therapies a routine part of medicine for the first time.
In some cases, the method will be used to switch off rogue genes that promote conditions such as heart failure or cancer. In others, it will help to activate genes that protect against nerve damage or encourage blood vessel growth.
In treating HIV, the aim is to modify T-cells from patients’ immune systems so that they become immune to infection with the virus. This would leave them with some working T-cells with which to fight off other infections, which are the chief cause of Aids deaths. The technique relies on a natural process by which the activity of genes is raised or lowered by proteins called transcription factors.
In 1985 Sir Aaron discovered a new class of proteins that mimic this function and can recognise specific stretches of DNA and bind to them, boosting the activity of genes or damping them down.
He named them zinc-finger proteins, after the metal that holds them together and the way in which they grasp DNA. Sangamo BioSciences, a company in California, has already developed several drugs based on the principle.
A zinc-finger protein specific to a gene is loaded with an enzyme called a nuclease, which will bind to the gene and turn it on or off. Sir Aaron told The Times: “We are taking nature’s own method of regulating gene activity and exploiting it for our own purposes. We can use this technique to change the function of a single gene permanently.
“The beauty of zinc-finger nucleases lies in their simplicity. Where other methods are long, arduous and often messy, it is relatively easy to switch off genes using this method. The zinc-finger design allows us to target a single gene, while the nuclease disrupts the gene.”
Details of the technique are published today in the journal Proceedings of the National Academy of Sciences.http://www.pnas.org/cgi/content/abstract/0800940105v1?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=Aaron+Klug&searchid=1&FIRSTINDEX=0&sortspec=date&resourcetype=HWCIT
The most advanced of Sangamo’s drugs uses a zinc-finger nuclease to treat diabetic neuropathy, a common complication of diabetes that causes nerve damage and pain. The drug binds to a gene called VEGF-A, which is known to protect the nervous system, and switches it on to prevent nerve damage. Phase 2 trials of the drug are under way. The same gene is also being targeted to treat peripheral arterial disease which causes blocked arteries in the limbs. A zinc-finger drug that has started safety trials aims to stimulate VEGF-A activity, which can promote the growth of new arteries.
In the longer term, a similar approach might be used to grow new blood vessels in the heart, Sir Aaron said.
Sangamo is applying for regulatory permission to start testing a zinc-finger nuclease on HIV patients as well as developing drugs to treat glioblastoma, a type of brain cancer, and single-gene disorders such as sickle-cell anaemia. 本人认领，48小时内若没有给出译文，请其它战友认领！ How zinc-finger proteins treat illness by aiming at a single gene
锌指蛋白针对单个基因的疾病治疗方法探究
From The TimesMarch 18, 2008
来源：泰晤士报 2008-3-18
A new way of turning genes on and off, pioneered by a Nobel prize-winning British scientist, is promising to transform treatment of conditions such as HIV/Aids, heart disease and diabetes.
一种新的基因调控方法（激活或者沉默基因）有望改变我们当前针对诸如爱滋病、心脏病以及糖尿病等疾病的治疗方法；该方法最先由一位获得诺贝尔奖的英国科学家提出。
The technique, devised by Sir Aaron Klug, of the Laboratory of Molecular Biology in Cambridge, allows scientists to act with unprecedented precision against genes that affect a wide range of diseases, switching them on or off permanently.
由剑桥大学分子生物学实验室的Aaron Klug先生发明的这项技术允许科学家们针对那些影响许多疾病的基因进行前所未有的精确操作，其中包括永久地激活或者沉默这些基因。
The first drugs designed to target the genes have begun clinical trials in the United States on patients with arterial disease and diabetes-induced nerve damage. A third trial, for HIV, is due to begin within months. If they are successful, scientists predict that the technique could change the way many diseases are treated, making genetic therapies a routine part of medicine for the first time.
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作者:admin@医学,生命科学 2010-12-23 17:11
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