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【每日动态】氢气对全脑缺血的治疗作用
本文将发表在外科研究杂志。Journal of Surgical Research, In Press, Uncorrected Proof, Available online 2 March 2011
The Effect of Hydrogen-Rich Saline on the Brain of Rats with Transient Ischemia.pdf
Qing Ji, Kangli Hui, Lidong Zhang, Xuejun Sun, Weiyan Li, Manlin Duan
Background
Due to its antioxidant and anti-inflammatory properties, hydrogen gas (H2) has protective effects on a variety of organs from damage induced by ischemia/reperfusion (I/R). In this study, we tested the protective effect of hydrogen-rich saline on the brain in a global cerebral I/R model.
Materials and Methods
We used a four-vessel occlusion model of global cerebral ischemia (15 min) and reperfusion, with rats. The rats were divided into four groups (n = 96: sham, I/R plus physiologic saline injected intraperitoneally, I/R plus hydrogen-rich saline injected intraperitoneally at the beginning of reperfusion, and I/R plus hydrogen-rich saline injected intraperitoneally 6 h after reperfusion began. One group of rats was sacrificed after 24 h of reperfusion. Malondialdehyde (MDA) was measured to quantify the oxidative stress. Capspase-3 was measured to indicate the status of apoptosis. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and nuclear factor-κB (NF-κB) were measured to monitor the inflammation. Another group of rats was sacrificed after 72 h of reperfusion to measure the histologic damages in hippocampus by hematoxylin and eosin staining and Nissl staining.
Results
Compared with rats with I/R only, hydrogen-rich saline treatment significantly improved the amount of surviving cells. NF-κB, TNF-α, IL-6, MDA, and caspase-3 were all increased significantly by I/R injury. Hydrogen-rich saline reduced all these markers.
Conclusions
Our data demonstrate that intraperitoneal injection of hydrogen-rich saline has strong protective effect on the transient global cerebral ischemia-reperfusion rats. 23页 山东大学文件类型:DOC/Microsoft Word- 文字版还拥有3个国家基础科学研究和教学人才培养基地海洋学、海洋化学、生命科学与技术. ... 且能明显改善神经症状,治疗脑缺血疗效显著;另外该药毒副作用小、价格低廉,具有较强的...lninfo.gov.cn/...103933833.doc-2010-10-25-清华大学科技成果重点推广项目文件类型:PDF/Adobe Acrobat- 文字版(4)治疗优越性:见效快、副作用小、较低成本. (5)环境友好:本设备对周围环境不造成影... ATP 的关键作用酶,具有抗氧化,提高细胞活 性和延长细胞周期的作用,是现代生命科学研...www.kfb.tsinghua.edu.cn/...pdf-2011-03-03-目录文件类型:DOC/Microsoft Word- 文字版红外锁定跟踪摄像系统(浙江大学) 5 多DSP并行处理实时动态信号分析系统(浙江大学) 5 ... 应用领域:生物物理学、生命科学、材料科学、基础物理学、基础化学等领域. 技术指标:...www.yzinfo.gov.cn/...技成果汇编.doc-2011-02-15-[标签:content2]
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作者:admin@医学,生命科学 2011-03-06 23:01
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